钙蛋白酶2催化亚基

钙蛋白酶2催化亚基
已知的结构
PDB	直系同源搜索：PDBe RCSB
PDBID列表
	1KFU、1KFX、2NQA
标识符
代号	CAPN2
扩展标识	OMIM：114230；MGI：88264；HomoloGene：1326；GeneCards：CAPN2；OMA：CAPN2 - orthologs
基因位置（人类）
染色体	1號染色體
终止	223,776,018 bp
基因位置（小鼠）
染色体	小鼠1号染色体
终止	182,345,173 bp
RNA表达模式
	Top expressed in
	bronchial epithelial cell; ; nasal epithelium; ; mucosa of sigmoid colon; ; right uterine tube; ; metanephric glomerulus; ; cartilage tissue;
	Top expressed in
	endothelial cell of lymphatic vessel; ; facial motor nucleus; ; left lung; ; tail of embryo; ; right lung; ; right lung lobe;
	查阅更多表达数据
	查阅更多表达数据
基因本體
分子功能	calcium ion binding; 半胱氨酸型肽酶活性; 金屬離子結合; cytoskeletal protein binding; 肽酶活性; 血浆蛋白结合; protein heterodimerization activity; 水解酶活性; calcium-dependent cysteine-type endopeptidase activity; 酶结合;
細胞組分	高尔基体; 偽足; 膜; 粘着斑; perinuclear endoplasmic reticulum; 胞內; 树突; 内质网; cortical actin cytoskeleton; 脂筏模型; 染色质; 溶酶体; 外排體; 细胞核; 细胞质基质; 細胞質; 细胞膜; mitochondrial intermembrane space; external side of plasma membrane; cell projection; soma;
生物學過程	response to hypoxia; protein autoprocessing; 蛋白酶解; cellular response to amino acid stimulus; myoblast fusion; proteolysis involved in cellular protein catabolic process; regulation of cytoskeleton organization; blastocyst development; extracellular matrix disassembly; female pregnancy; positive regulation of cardiac muscle cell apoptotic process; regulation of interleukin-6 production; cellular response to interferon-beta; response to hydrogen peroxide; behavioral response to pain; cellular response to lipopolysaccharide; positive regulation of neuron death; positive regulation of myoblast fusion; positive regulation of phosphatidylcholine biosynthetic process;
	来源：Amigo / QuickGO
直系同源
	824
	12334
	ENSG00000162909
	ENSMUSG00000026509
	P17655
	O08529
	NM_001146068; NM_001748
	NM_009794
	NP_001139540; NP_001739
	NP_033924
	维基数据
檢視/編輯人類	檢視/編輯小鼠

钙蛋白酶2催化亚基（英语：Calpain-2 catalytic subunit）是人类中由CAPN2基因编码的蛋白质。^[5]^[6]

功能

钙蛋白酶是钙激活的中性蛋白酶，是非溶酶体的细胞内半胱氨酸蛋白酶。哺乳动物钙蛋白酶包括普遍存在的胃特异性和肌肉特异性蛋白质。普遍存在的酶由异二聚体组成，这些异二聚体具有与常见的小调节亚基相关的独特的大催化亚基。该基因编码普遍存在的酶钙蛋白酶2的大亚基。已报道5' UTR中存在多个异质转录起始位点。^[7]

相互作用

CAPN2已被证明与Bcl-2产生相互作用。^[8]

参考资料

^ ^1.0 ^1.1 ^1.2 GRCh38: Ensembl release 89: ENSG00000162909 – Ensembl, May 2017
^ ^2.0 ^2.1 ^2.2 GRCm38: Ensembl release 89: ENSMUSG00000026509 – Ensembl, 2017年5月
^ Human PubMed Reference:. National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Mouse PubMed Reference:. National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Imajoh S, Aoki K, Ohno S, Emori Y, Kawasaki H, Sugihara H, Suzuki K. Molecular cloning of the cDNA for the large subunit of the high-Ca2+-requiring form of human Ca2+-activated neutral protease. Biochemistry. 1988, 27 (21): 8122–8. PMID 2852952. doi:10.1021/bi00421a022.
^ Hata A, Ohno S, Akita Y, Suzuki K. Tandemly reiterated negative enhancer-like elements regulate transcription of a human gene for the large subunit of calcium-dependent protease. J. Biol. Chem. May 1989, 264 (11): 6404–11. PMID 2539381. doi:10.1016/S0021-9258(18)83364-6 .
^ Entrez Gene: CAPN2 calpain 2, (m/II) large subunit. [2024-01-10]. （原始内容存档于2021-05-07）.
^ Gil-Parrado S, Fernández-Montalván A, Assfalg-Machleidt I, Popp O, Bestvater F, Holloschi A, Knoch TA, Auerswald EA, Welsh K, Reed JC, Fritz H, Fuentes-Prior P, Spiess E, Salvesen GS, Machleidt W. Ionomycin-activated calpain triggers apoptosis. A probable role for Bcl-2 family members. J. Biol. Chem. Jul 2002, 277 (30): 27217–26. PMID 12000759. doi:10.1074/jbc.M202945200 .

延伸阅读

Suzuki K, Sorimachi H, Yoshizawa T, Kinbara K, Ishiura S. Calpain: novel family members, activation, and physiologic function. Biol. Chem. Hoppe-Seyler. 1995, 376 (9): 523–9. PMID 8561910. doi:10.1515/bchm3.1995.376.9.523.
Cohen GM. Caspases: the executioners of apoptosis. Biochem. J. 1997, 326 (Pt 1): 1–16. PMC 1218630 . PMID 9337844. doi:10.1042/bj3260001.
Reverter D, Sorimachi H, Bode W. The structure of calcium-free human m-calpain: implications for calcium activation and function. Trends Cardiovasc. Med. 2001, 11 (6): 222–9. PMID 11673052. doi:10.1016/S1050-1738(01)00112-8.
Kopp S. Reproducibility of response to a questionnaire on symptoms of masticatory dysfunction. Community Dent Oral Epidemiol. 1976, 4 (5): 205–9. PMID 1067155. doi:10.1111/j.1600-0528.1976.tb00985.x.
Adachi Y, Kitahara-Ozawa A, Sugamura K, Lee WJ, Yodoi J, Maki M, Murachi T, Hatanaka M. Expression of calpain II gene in human hematopoietic system cells infected with human T-cell leukemia virus type I. J. Biol. Chem. 1992, 267 (27): 19373–8. PMID 1527057. doi:10.1016/S0021-9258(18)41785-1 .
Ohno S, Minoshima S, Kudoh J, Fukuyama R, Shimizu Y, Ohmi-Imajoh S, Shimizu N, Suzuki K. Four genes for the calpain family locate on four distinct human chromosomes. Cytogenet. Cell Genet. 1990, 53 (4): 225–9. PMID 2209092. doi:10.1159/000132937.
Ishiguro H, Higashiyama S, Namikawa C, Kunimatsu M, Takano E, Tanaka K, Ohkubo I, Murachi T, Sasaki M. Interaction of human calpains I and II with high molecular weight and low molecular weight kininogens and their heavy chain: mechanism of interaction and the role of divalent cations. Biochemistry. 1987, 26 (10): 2863–70. PMID 3038169. doi:10.1021/bi00384a030.
Srinivasula SM, Fernandes-Alnemri T, Zangrilli J, Robertson N, Armstrong RC, Wang L, Trapani JA, Tomaselli KJ, Litwack G, Alnemri ES. The Ced-3/interleukin 1beta converting enzyme-like homolog Mch6 and the lamin-cleaving enzyme Mch2alpha are substrates for the apoptotic mediator CPP32. J. Biol. Chem. 1996, 271 (43): 27099–106. PMID 8900201. doi:10.1074/jbc.271.43.27099 .
Corasaniti MT, Navarra M, Catani MV, Melino G, Nisticò G, Finazzi-Agrò A. NMDA and HIV-1 coat protein, GP120, produce necrotic but not apoptotic cell death in human CHP100 neuroblastoma cultures via a mechanism involving calpain. Biochem. Biophys. Res. Commun. 1996, 229 (1): 299–304. PMID 8954122. doi:10.1006/bbrc.1996.1796.
Fujitani K, Kambayashi J, Sakon M, Ohmi SI, Kawashima S, Yukawa M, Yano Y, Miyoshi H, Ikeda M, Shinoki N, Monden M. Identification of mu-, m-calpains and calpastatin and capture of mu-calpain activation in endothelial cells. J. Cell. Biochem. 1997, 66 (2): 197–209. PMID 9213221. S2CID 85163404. doi:10.1002/(SICI)1097-4644(19970801)66:2<197::AID-JCB7>3.0.CO;2-L.
Rock MT, Brooks WH, Roszman TL. Calcium-dependent signaling pathways in T cells. Potential role of calpain, protein tyrosine phosphatase 1b, and p130Cas in integrin-mediated signaling events. J. Biol. Chem. 1997, 272 (52): 33377–83. PMID 9407132. doi:10.1074/jbc.272.52.33377 .
Ueyama H, Kumamoto T, Fujimoto S, Murakami T, Tsuda T. Expression of three calpain isoform genes in human skeletal muscles. J. Neurol. Sci. 1998, 155 (2): 163–9. PMID 9562261. S2CID 205898607. doi:10.1016/S0022-510X(97)00309-2.
Strobl S, Fernandez-Catalan C, Braun M, Huber R, Masumoto H, Nakagawa K, Irie A, Sorimachi H, Bourenkow G, Bartunik H, Suzuki K, Bode W. The crystal structure of calcium-free human m-calpain suggests an electrostatic switch mechanism for activation by calcium. Proc. Natl. Acad. Sci. U.S.A. 2000, 97 (2): 588–92. Bibcode:2000PNAS...97..588S. PMC 15374 . PMID 10639123. doi:10.1073/pnas.97.2.588 .
Masumoto H, Nakagawa K, Irie S, Sorimachi H, Suzuki K, Bourenkov GP, Bartunik H, Fernandez-Catalan C, Bode W, Strobl S. Crystallization and preliminary X-ray analysis of recombinant full-length human m-calpain. Acta Crystallogr. D. 2000, 56 (Pt 1): 73–5. Bibcode:2000AcCrD..56...73M. PMID 10666632. doi:10.1107/S0907444999013748.
Chua BT, Guo K, Li P. Direct cleavage by the calcium-activated protease calpain can lead to inactivation of caspases. J. Biol. Chem. 2000, 275 (7): 5131–5. PMID 10671558. doi:10.1074/jbc.275.7.5131 .
Lee MS, Kwon YT, Li M, Peng J, Friedlander RM, Tsai LH. Neurotoxicity induces cleavage of p35 to p25 by calpain. Nature. 2000, 405 (6784): 360–4. Bibcode:2000Natur.405..360L. PMID 10830966. S2CID 205006589. doi:10.1038/35012636.

外部链接

The MEROPS online database for peptidases and their inhibitors: C02.002 （页面存档备份，存于互联网档案馆）
Human CAPN2 genome location and CAPN2 gene details page in the UCSC Genome Browser.

[refGRCh38Ensembl-1] 1.0 ^1.1 ^1.2 GRCh38: Ensembl release 89: ENSG00000162909 – Ensembl, May 2017

[refGRCm38Ensembl-2] 2.0 ^2.1 ^2.2 GRCm38: Ensembl release 89: ENSMUSG00000026509 – Ensembl, 2017年5月

[3] Human PubMed Reference:. National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] Mouse PubMed Reference:. National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid2852952-5] Imajoh S, Aoki K, Ohno S, Emori Y, Kawasaki H, Sugihara H, Suzuki K. Molecular cloning of the cDNA for the large subunit of the high-Ca2+-requiring form of human Ca2+-activated neutral protease. Biochemistry. 1988, 27 (21): 8122–8. PMID 2852952. doi:10.1021/bi00421a022.

[pmid2539381-6] Hata A, Ohno S, Akita Y, Suzuki K. Tandemly reiterated negative enhancer-like elements regulate transcription of a human gene for the large subunit of calcium-dependent protease. J. Biol. Chem. May 1989, 264 (11): 6404–11. PMID 2539381. doi:10.1016/S0021-9258(18)83364-6 .

[entrez-7] Entrez Gene: CAPN2 calpain 2, (m/II) large subunit. [2024-01-10]. （原始内容存档于2021-05-07）.

[pmid12000759-8] Gil-Parrado S, Fernández-Montalván A, Assfalg-Machleidt I, Popp O, Bestvater F, Holloschi A, Knoch TA, Auerswald EA, Welsh K, Reed JC, Fritz H, Fuentes-Prior P, Spiess E, Salvesen GS, Machleidt W. Ionomycin-activated calpain triggers apoptosis. A probable role for Bcl-2 family members. J. Biol. Chem. Jul 2002, 277 (30): 27217–26. PMID 12000759. doi:10.1074/jbc.M202945200 .

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蛋白酶类：半胱氨酸蛋白酶类（EC 3.4.22)
胱天蛋白酶	胱天蛋白酶1 胱天蛋白酶2 胱天蛋白酶3 胱天蛋白酶4 胱天蛋白酶5 胱天蛋白酶6 胱天蛋白酶7 胱天蛋白酶8 胱天蛋白酶9 胱天蛋白酶10 胱天蛋白酶11 胱天蛋白酶12 胱天蛋白酶13 胱天蛋白酶14
来自果实	木瓜蛋白酶无花果蛋白酶鳳梨蛋白酶猕猴桃蛋白酶
钙蛋白酶	CAPN1 CAPN2 CAPN3 CAPN5 CAPN6 CAPN7 CAPN8 CAPN9 CAPN10 CAPN11 CAPN12 CAPN13 CAPN14 CAPNS1 CAPNS2
组织蛋白酶	B C F H K L1/L2 O S T W Z
其它	梭菌蛋白酶癌性促凝物质分离酶自噬蛋白酶 Cruzipain 3C样蛋白酶牙龈蛋白酶R 牙龈蛋白酶K
EC 1.1/2/3/4/5/6/7/8/9/10/11/12/13/14/15/16/17/18/19/20/21/22 · 2.1/2/3/4/5/6/7(2.7.10/11-12)/8/9 · 3.1/2/3/4(3.4.21/22/23/24)/5/6/7/8/9/10/11/12/13 · 4.1/2/3/4/5/6 · 5.1/2/3/4/5/99 · 6.1-3/4/5-6